DCFH-DA 活性氧ROS荧光探针
2′,7′-Dichlorodihydrofluorescein Diacetate
CAS: 4091-99-0
Molecular Formula: C24H14Cl2O7
DCFH-DA 活性氧ROS荧光探针 - Names and Identifiers
| Name | 2′,7′-Dichlorodihydrofluorescein Diacetate |
| Synonyms | Dichlorofluorescin diacetate 2'',7''-Dichlorofluorescein diac 2,7-Dichlorodihydrofluorescein diacetate 2′,7′-Dichlorodihydrofluorescein Diacetate 2-(2,7-Dichloro-3,6-diacetoxy-9H-xanthen-9-yl)benzoic acid |
| CAS | 4091-99-0 |
| EINECS | 685-843-8 |
DCFH-DA 活性氧ROS荧光探针 - Physico-chemical Properties
| Molecular Formula | C24H14Cl2O7 |
| Molar Mass | 485.27 |
| Density | 1.463±0.06 g/cm3(Predicted) |
| Melting Point | 209-210 °C(lit.) |
| Boling Point | 593.5±50.0 °C(Predicted) |
| Solubility | Solubility in water: insoluble Solubility in other solvents: soluble in DMSO, alcohols |
| Appearance | Morphological Crystalline solid |
| pKa | 3.79±0.36(Predicted) |
| Storage Condition | -20°C |
| Sensitive | Light and humidity sensitivity |
| MDL | MFCD00037501 |
| Physical and Chemical Properties | Bioactive H2DCFDA (DCFH-DA, 2 ',7'-Dichlorodihydrofluorescein diacetate) is a cell-permeable probe that can be used to detect the production of intracellular reactive oxygen species (ROS). |
| Use | Application 2 ',7'-dichlorofluorescein diacetate is a fluorescent probe. As a non-polar compound, it can quickly diffuse into the cell. The esterase in the cell hydrolyzes it to the non-fluorescent dichlorofluorescein (DCFH), so that the probe is loaded into the cell. |
DCFH-DA 活性氧ROS荧光探针 - Risk and Safety
| Hazard Symbols | Xi - Irritant |
| Risk Codes | 36/37/38 - Irritating to eyes, respiratory system and skin. |
| Safety Description | S22 - Do not breathe dust. S24/25 - Avoid contact with skin and eyes. S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. |
| WGK Germany | 3 |
| FLUKA BRAND F CODES | 10-21 |
| HS Code | 29329990 |
DCFH-DA 活性氧ROS荧光探针 - Upstream Downstream Industry
| Raw Materials | 2',7'-DICHLOROFLUORESCIN DIACETATE |
| Downstream Products | 2',7'-DICHLOROFLUORESCEIN 2',7'-dichlorodihydrofluorescein |
DCFH-DA 活性氧ROS荧光探针 - Reference
| Reference Show more | 1. Hu Menghua, Yan Xiaoxiao, Huang Zhixian, etc. Identification of a wild bamboo yellow fungus and optimization of fermentation conditions for extraction and fermentation of hypocrellin a [J]. Journal of edible fungi 2020 027(001):49-62. 2. Jin master, Hong Yi, Lu Shan, Li Danqing, Cao Yan. Toxicity of Ethanol extracts of Panax notoginseng with different particle sizes on Caenorhabditis elegans [J]. Journal of Toxicology, 2019, 33(03):208-212. 3. Jin, Si-Yi, et al. "Ethanol extracts of Panax notoginsen incremental lifetime and protect against oxidative stress." Journal of Functional Foods 58 (2019): 218-226.https:// doi.org/10.1016/j.jf 4. [IF = 16.806] Yajuan Liu et al."Boosting Antitumor Sonodynamic Therapy Efficacy of Black Phosphorus via Covalent Functionalization."Adv Sci. 2021 Oct;8(20):2102422 5. [IF=9.229] Yadong Li et al."Carbon Dots as a Protective Agent Alleviating Abiotic Stress on Rice (Oryza sativa L.) through Promoting Nutrition Assimilation and the Defense System."Acs Appl Mater Inter. 2020;12(30):33575-33585 6. [IF=8.579] Hongxia Tang et al."Targeted Manganese doped silica nano GSH-cleaner for treatment of Liver Cancer by destroying the intracellular redox homeostasis."Theranostics. 2020; 10(21): 9865-9887 7. [IF=7.79] Weidong Fei et al."Targeted GSH-exhausting and hydroxyl radical self-producing manganese-silica nanomissiles for MRI guided ferroptotic cancer therapy."Nanoscale. 2020 Aug;12(32):16738-16754 8. [IF=7.514] Tao Bao et al."Cold plasma: An emerging pretreatment technology for the drying of jujube slices."Food Chem. 2021 Feb;337:127783 9. [IF=6.953] Zhou Dong et al."Purification and comparative study of bioactivities of a natural selenized polysaccharide from Ganoderma Lucidum mycelia."Int J Biol Macromol. 2021 Nov;190:101 10. [IF=5.878] Li DanQing et al."N-butanol extract of Hedyotis diffusa protects transgenic Caenorhabditis elegans from Aβ-induced toxicity."Phytother Res. 2021 Feb;35(2):1048-1061 11. [IF=5.279] Yun-Feng Zheng et al."Comprehensive Comparison of Two Color Varieties of Perillae Folium Using Rapid Resolution Liquid Chromatography Coupled with Quadruple-Time-of-Flight Mass Spectrometry (RRLC-Q/TOF-MS)-Based Metabolic Profile and in Vivo/in Vitro Anti 12. [IF=5.162] Huibin Dong et al."Polysaccharide SAFP from Sarcodon aspratus attenuates oxidative stress-induced cell damage and bleomycin-induced pulmonary fibrosis."Int J Biol Macromol. 2020 Dec;164:1215 13. [IF=4.36] Qian Hu et al."Antioxidant capacity of flavonoids from Folium Artemisiae Argyi and the molecular mechanism in Caenorhabditis elegans."J Ethnopharmacol. 2021 Oct;279:114398 14. [IF=2.753] Nabila Jahan et al."Neferine improves oxidative stress and apoptosis in benign prostate hyperplasia via Nrf2-ARE pathway.."Redox Rep. 2021;26(1):1-9 15. [IF=11.413] Xiaojie Chen et al."Cooperative coordination-mediated multi-component self-assembly of “all-in-one” nanospike theranostic nano-platform for MRI-guided synergistic therapy against breast cancer."Acta Pharm Sin B. 2022 Feb;: 16. [IF=13.281] Ziyu Wu et al."Multi-Pathway Microenvironment Regulation for Atherosclerosis Therapy Based on Beta-Cyclodextrin/L-Arginine/Au Nanomotors with Dual-Mode Propulsion."Small. 2021 Dec 16 |
DCFH-DA 活性氧ROS荧光探针 - Nature
| Solubility | DMF: soluble |
| BRN | 4610273 |
| EPA chemical information | 2',7'-Dichlorohydrofluorescein diacetate (4091-99-0) |
Last Update：2024-04-10 22:29:15
DCFH-DA 活性氧ROS荧光探针 - Uses and synthesis methods
Target
Target Value
ROS
in vitro studies
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. H2DCFDA is dissolved in DMSO to obtain a 10 mM stock solutions and further diluted before use.
2. Cells are incubated with 5 µM H2DCFDA solution in PBS in the dark for 30 min at 37°C, then harvested with 0.05% trypsin-EDTA solution, suspended in a fresh medium, and immediately analyzed with flow cytometer.
3. Along with the H2DCFDA probe, if indicated, use ROS-insensitive modification of the fluorescein dye DCFDA as a positive control. The staining procedure is the same as for the H2DCFDA.
Last Update：2024-04-10 22:29:15
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Raw Materials for DCFH-DA 活性氧ROS荧光探针
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